http://honig.c2b2.columbia.edu/cryoem Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the te…
Predicting protein structure from cryo-EM data
WebMar 31, 2024 · The 3D cryoEM density maps generated in this study have been deposited in the Electron Microscopy Data Bank (EMDB) under accession codes EMD-13319, EMD-13320, and EMD-13325. The atomic coordinates have been deposited in the Protein Data Bank, with accession codes 7PCF, 7PCH, and 7PCQ. All study data are included in the … WebNov 18, 2024 · The density maps of V 1 -ATPase, β-galactosidase, and apoferritin were also reconstructed at 3.03, 1.81, and 1.29 Å resolution, respectively. These results indicate that the EG-grid will be a powerful tool for high-throughput cryoEM data collection to accelerate high-resolution structural analysis of biological macromolecules. fawcitt consulting
From structure to sequence: Antibody discovery using cryoEM
WebMay 1, 2024 · Cryo electron microscopy sample preparation flow chart. Sample preparation flowchart for cryoEM in which choices (grey) for several preparation and visualization techniques (blue) depend on: (i) the sample thickness, and (ii) … WebCryoEM In-Residence Training and Public Lecture: Tomography and Volta Phase Plate S2C2 CryoEM CCP-EM Modeling Workshop SSRL/LCLS Users' Meeting: CryoEM of COVID-related Macromolecules Fundamentals of Single Particle CryoEM Workshop S2C2 CryoEM Image Processing Workshop: cryoSPARC WebMay 1, 2024 · Cryo electron microscopy sample preparation flow chart. Sample preparation flowchart for cryoEM in which choices (grey) for several preparation and visualization … faw circle 2